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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
02/03/2017 |
Actualizado : |
07/03/2017 |
Autor : |
GARCIA, J.P; GIANNITTI, F.; FINNIE, J.W.; MANAVIS, J. .; BEINGESSER, J.; ADAMS ,V.; ROOD, J.I.; UZAL, F.A. |
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Comparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Veterinary Pathology, 2015, v.52.n.6. p.1250-1253, 2015. |
Idioma : |
Inglés |
Contenido : |
Abstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease. MenosAbstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated wi... Presentar Todo |
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ENFERMEDADES DE LOS ANIMALES. |
Asunto categoría : |
-- |
Marc : |
LEADER 02512naa a2200217 a 4500 001 1056750 005 2017-03-07 008 2015 bl uuuu u00u1 u #d 100 1 $aGARCIA, J.P 245 $aComparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives.$h[electronic resource] 260 $c2015 520 $aAbstract Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease. 650 $aENFERMEDADES DE LOS ANIMALES 700 1 $aGIANNITTI, F. 700 1 $aFINNIE, J.W. 700 1 $aMANAVIS, J. . 700 1 $aBEINGESSER, J. 700 1 $aADAMS ,V. 700 1 $aROOD, J.I. 700 1 $aUZAL, F.A. 773 $tVeterinary Pathology, 2015$gv.52.n.6. p.1250-1253, 2015.
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
30/10/2019 |
Actualizado : |
31/01/2020 |
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
Autor : |
MIGUEZ, M.; DELEÓN, R.; VICENTE, G.; ZOPPOLO, R. |
Afiliación : |
MATIAS MIGUEZ, Departamento de Ingeniería Eléctrica, Universidad Católica del Uruguay; RUBEN DELEÓN, Departamento de Ingeniería Eléctrica, Universidad Católica del Uruguay; GABRIEL VICENTE, Departamento de Ingeniería Eléctrica, Universidad Católica del Uruguay; ROBERTO JOSE ZOPPOLO GOLDSCHMIDT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Real time tree row volume estimation for efficient application of phytosanitary products in fruit trees. (Conference Paper). |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
Proceedings - IEEE International Symposium on Circuits and Systems (ISCAS), 2019, Volume 2019-May, 2019, Article number 8702561. |
ISSN : |
2158-1525 |
DOI : |
10.1109/ISCAS.2019.8702561 |
Idioma : |
Inglés |
Notas : |
2019 IEEE International Symposium on Circuits and Systems, ISCAS 2019; Sapporo Convention CenterSapporo; Japan; 26 May 2019 through 29 May 2019; Category numberCFP19ISC-ART; Code 148242. |
Contenido : |
ABSTRACT.
Fruit cultivation in Uruguay are done mainly by small-scale producers, where a single sprayer is used for all the phytosanitary applications. For proper protection of the fruit trees, the broth concentration (the phytosanitary diluted in water) must be adjusted according to the volume of vegetation of the trees. In most farms, this concentration calibration is done once per year for the worst case scenario. Therefore, excessive doses of pesticides are applied to the trees with lower vegetative mass. This over application increases the cost of production and can also potentially pose risks for the consumers and/or environment. A low cost system adaptable to a standard sprayer capable of measuring the Tree Row Volume (TRV) in real time and adjusting the corresponding dose was developed. Initial field tests showed a reduction of between 30%-60% for different fruit trees, reducing the environmental risk of contamination, lowering cost and lowering the risk of pesticide residues on fruit for the final consumer. The system also includes a GPS, to deduct from the position the kind of tree to be sprayed and serving as a data logger for better traceability of past applications. Further research in the use of a multi-sensor solution, as well as extensive testing in real conditions is being conducted. © 2019 IEEE |
Palabras claves : |
Orchards; Phytosanitary application; Tree row volume. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02275nam a2200217 a 4500 001 1060366 005 2020-01-31 008 2019 bl uuuu u01u1 u #d 022 $a2158-1525 024 7 $a10.1109/ISCAS.2019.8702561$2DOI 100 1 $aMIGUEZ, M. 245 $aReal time tree row volume estimation for efficient application of phytosanitary products in fruit trees. (Conference Paper).$h[electronic resource] 260 $aProceedings - IEEE International Symposium on Circuits and Systems (ISCAS), 2019, Volume 2019-May, 2019, Article number 8702561.$c2019 500 $a2019 IEEE International Symposium on Circuits and Systems, ISCAS 2019; Sapporo Convention CenterSapporo; Japan; 26 May 2019 through 29 May 2019; Category numberCFP19ISC-ART; Code 148242. 520 $aABSTRACT. Fruit cultivation in Uruguay are done mainly by small-scale producers, where a single sprayer is used for all the phytosanitary applications. For proper protection of the fruit trees, the broth concentration (the phytosanitary diluted in water) must be adjusted according to the volume of vegetation of the trees. In most farms, this concentration calibration is done once per year for the worst case scenario. Therefore, excessive doses of pesticides are applied to the trees with lower vegetative mass. This over application increases the cost of production and can also potentially pose risks for the consumers and/or environment. A low cost system adaptable to a standard sprayer capable of measuring the Tree Row Volume (TRV) in real time and adjusting the corresponding dose was developed. Initial field tests showed a reduction of between 30%-60% for different fruit trees, reducing the environmental risk of contamination, lowering cost and lowering the risk of pesticide residues on fruit for the final consumer. The system also includes a GPS, to deduct from the position the kind of tree to be sprayed and serving as a data logger for better traceability of past applications. Further research in the use of a multi-sensor solution, as well as extensive testing in real conditions is being conducted. © 2019 IEEE 653 $aOrchards 653 $aPhytosanitary application 653 $aTree row volume 700 1 $aDELEÓN, R. 700 1 $aVICENTE, G. 700 1 $aZOPPOLO, R.
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